Cat. #153527
NUOC-1 Cell Line
Cat. #: 153527
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Richard Edmondson
Institute: Northern Institute For Cancer Research, Newcastle University
Tool Details
*FOR RESEARCH USE ONLY
- Name: NUOC-1 Cell Line
- Alternate name: HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer
- Tool sub type: Continuous
- Parental cell: Ascites of chemotherapy naive patient
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Growth properties: Doubling time approximately 58 hours
- Model: Cancer Model
- Conditional: No
- Description: Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 3039%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy na
ve patient with a primary mixed endometrioid/ clear cell/ high grade serous ... - Production details: Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37ÄÂ?°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence
- Biosafety level: 1
Target Details
- Target: Ovarian cancer
Applications
- Application notes: NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.
- Functional characterisation of a novel ovarian cancer cell line, NUOC-1.
