Anti-IFN-γrel [VAB4P2B4*E8]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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![Western blot data of recombinant IFN-γrel probed with Anti-IFN-γrel [VAB4P2B4*E8]. A 22 kDa band confirms target recognition.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/b2549bed-c9de-4f5a-bd6c-eb9ee4c7c4ae.png)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Weak immunostaining is observed in spleen of Atlantic Salmon using Anti-IFN-γrel [VAB4P2B4*E8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/99d6278b-1953-4ac0-94d3-1ed83e22b2de.png)
![Western blot data of recombinant IFN-γrel probed with Anti-IFN-γrel [VAB4P2B4*E8]. A 22 kDa band confirms target recognition.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/b2549bed-c9de-4f5a-bd6c-eb9ee4c7c4ae.png)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Weak immunostaining is observed in spleen of Atlantic Salmon using Anti-IFN-γrel [VAB4P2B4*E8]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/99d6278b-1953-4ac0-94d3-1ed83e22b2de.png)
![Image thumbnail for Anti-IFN-γrel [VAB4P2B4*E8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/b2549bed-c9de-4f5a-bd6c-eb9ee4c7c4ae.png)
![Image thumbnail for Anti-IFN-γrel [VAB4P2B4*E8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/99d6278b-1953-4ac0-94d3-1ed83e22b2de.png)
| Catalogue Number | 153451 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Anti-IFN-γrel |
| Synonyms | IFG antibody, IFI antibody, IFN gamma antibody, IFN immune antibody, IFN-gamma antibody, IFNG antibody, Immune interferon antibody, Interferon gamma antibody |
| Reactivity | Salmon and Brown Trout |
| Relevance | Interferons (IFNs) were originally identified as proteins that induce an antiviral state in cells, but they also have important regulatory functions in the immune system. Type I IFN (predominantly IFN-α and IFN-β) and type II IFN (IFN-γ) play critical roles in innate and adaptive immune response against viral infection in mammals. Unlike mammals fish have two type II interferons, IFN-γ and IFN-γ-related (IFN-γrel). IFN-γ and IFN-γrel have recently been shown to play a role in protecting particular species of fish against infection by pathogens such as infectious pancreatic necrosis virus (IPNV) and salmonid alphavirus 3 (SAV3). VAB4P2B4*E8 can be used to study IFN-γrel in Atlantic salmon (Salmo salar) and Brown trout (Salmo trutta). |
| Host | Mouse |
| Immunogen | ovalbumin-conjugated synthetic peptide-DLKRSYYQDR; peptide immunogen is 90% identical to rainbow trout (Oncorhynchus mykiss) |
| Positive Control | Western Blot-IFN-γrel recombinant; IHC- Formalin-fixed, paraffin-embedded fish head kidney and spleen |
| Subclass | IgG |
| Molecular Weight (kDa) | 22 kDa |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Recommended Usage Conditions: ELISA- neat, Western Blot- neat, IHC- 1/3, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
| Research Area | Aquaculture, Immunology |
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