Anti-ELOVL5 [Z88]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
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![Immunohistochemistry images are showing a strong cytoplasmic positivity of ELOVL5 in (A) primary colorectal cancer whilst weak staining is observed in (B) normal colon mucosa using Anti-ELOVL5 [Z88]. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/338cb92b-ef4a-401d-beb7-23fe2b6acc6f.png)
![Western blot data for Anti-ELOVL5 [Z88]. Band of expected size (34KDa) is observed in Jurkat cell lysates.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/771fcdc1-052a-4e6d-85a8-4b8ef548ca5c.png)
![Survival curve comparing patients outcome categorised by expression of ELOVL5 using Anti-ELOVL5 [Z88]. Strong expression of ELOVL5 was associated with improved patients’ outcome (p=0.006).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/11864ebe-e1cb-4533-b048-0a435fb863ae.png)
![Immunohistochemistry images are showing a strong cytoplasmic positivity of ELOVL5 in (A) primary colorectal cancer whilst weak staining is observed in (B) normal colon mucosa using Anti-ELOVL5 [Z88]. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/338cb92b-ef4a-401d-beb7-23fe2b6acc6f.png)
![Western blot data for Anti-ELOVL5 [Z88]. Band of expected size (34KDa) is observed in Jurkat cell lysates.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/771fcdc1-052a-4e6d-85a8-4b8ef548ca5c.png)
![Survival curve comparing patients outcome categorised by expression of ELOVL5 using Anti-ELOVL5 [Z88]. Strong expression of ELOVL5 was associated with improved patients’ outcome (p=0.006).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/11864ebe-e1cb-4533-b048-0a435fb863ae.png)
![Image thumbnail for Anti-ELOVL5 [Z88]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/338cb92b-ef4a-401d-beb7-23fe2b6acc6f.png)
![Image thumbnail for Anti-ELOVL5 [Z88]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/771fcdc1-052a-4e6d-85a8-4b8ef548ca5c.png)
![Image thumbnail for Anti-ELOVL5 [Z88]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/11864ebe-e1cb-4533-b048-0a435fb863ae.png)
| Catalogue Number | 153343 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | ELOVL Fatty Acid Elongase 5 |
| Synonyms | Elongation of very long chain fatty acids protein 5; ELOVL5; 3-keto acyl-CoA synthase ELOVL5; ELOVL FA elongase 5; Fatty acid elongase 1; Very long chain 3-ketoacyl-CoA synthase 5 |
| Reactivity | Human |
| Relevance | This protein is involved in the elongation of long-chain polyunsaturated fatty acids functioning as a condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. It may also participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. |
| Host | Mouse |
| Immunogen | Peptide Sequence –NNVKPRKLR (peptide immunogen is identical in primates) |
| Positive Control | Western Blot: Jurkat cell lysates. IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray. |
| Subclass | IgG |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC neat (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0). |
| Research Area | Cell Signaling & Signal Transduction, Cardiovascular |
References: 2 entries
The expression of brown fat-associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis.
Europe PMC ID: 30737786
The expression of brown fat associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis. Alnabulsi et al. 2019. Int J Cancer. :. PMID: 30737786.
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References: 2 entries
The expression of brown fat-associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis.
The expression of brown fat associated proteins in colorectal cancer and the relationship of uncoupling protein 1 with prognosis. Alnabulsi et al. 2019. Int J Cancer. :. PMID: 30737786.
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