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Wilms´ tumor 17.94 Cell Line is an anaplastic Wilms´ tumor (WT) cell line, which expressed NCAM, SALL1, and phenotypic features expected of metanephric blastema-derived cells. In Brown et al., 2005 treatment of 17.94 cells with 12-O-Tetradecanoylphorbol 13-acetate caused morphological changes, which led to reduced NCAM and SALL1 expression, but expression of vimentin was maintained.
Wilms´ tumor 17.94 Cell Line contains a TP53 mutation but lacks mutations in WT1, WTX, or CTNNB1, other genes involved in WT pathogenesis. The cells demonstrate no loss of heterozygosity at 7p, 11p, or 16q although DNA hypermethylation was detected at several loci, including the H19 differentially methylated region and at the PCDH@ gene clusters at 5q31.
|Production Details||Established from the nephrectomy specimen of a large lobulated Wilms´ tumor of a 4-year-old girl; cells were described to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation.|
|Recommended Growing Conditions||80% Dulbecco´s MEM + 20% h.i. FBS at 37 °C with 5% CO2. Seed out at ca. 0.5-1 x 10^6 cells/25 cm2 flask; split semi-confluent culture 1:2 once a week using TrypLE Express.|
|Notes||Freeze in 70% medium, 20% FBS, 10% DMSO|
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