Cat. #153333
Wilms’ tumour 17.94 Cell Line
Cat. #: 153333
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Kidney
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Keith Brown
Institute: University Of Bristol
Tool Details
*FOR RESEARCH USE ONLY
- Name: Wilms’ tumour 17.94 Cell Line
- Organism: Human
- Tissue: Kidney
- Model: Tumour line
- Conditional: No
- Description: Wilms´ tumor 17.94 Cell Line is an anaplastic Wilms´ tumor (WT) cell line, which expressed NCAM, SALL1, and phenotypic features expected of metanephric blastema-derived cells. In Brown et al., 2005 treatment of 17.94 cells with 12-O-Tetradecanoylphorbol 13-acetate caused morphological changes, which led to reduced NCAM and SALL1 expression, but expression of vimentin was maintained. Wilms´ tumor 17.94 Cell Line contains a TP53 mutation but lacks mutations in WT1, WTX, or CTNNB1, other genes involved in WT pathogenesis. The cells demonstrate no loss of heterozygosity at 7p, 11p, or 16q although DNA hypermethylation was detected at several loci, including the H19 differentially methylated region and at the PCDH@ gene clusters at 5q31.
- Production details: Established from the nephrectomy specimen of a large lobulated WilmsÄÂ?´ tumor of a 4-year-old girl; cells were described to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation. Freeze in 70% medium, 20% FBS, 10% DMSO
- Biosafety level: 1
- Cellosaurus id: CVCL_D704
Handling
- Format: Frozen
- Growth medium: 80% Dulbecco's MEM + 20% h.i. FBS at 37?°C with 5% CO2. Seed out at ca. 0.5-1 x 10^6 cells/25 cm2 flask; split semi-confluent culture 1:2 once a week using TrypLE Express.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage medium: 70% medium, 20% FBS, 10% DMSO
- Mycoplasma free: Yes
References
- Brown et al. 2012. Cancer Genet. 205(6):319-26. PMID: 22749038.
- Characterization of 17.94, a novel anaplastic Wilms' tumor cell line.
