Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody
Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
- Datasheet
- References (6)
- Inventor Info
Info
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/dd894089-cc88-43d6-ac08-3a7adf8e8fa6.jpg)
![Flow cytometry: Binding of 10 μg/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/379cbb01-0284-40b9-a6a6-f7f4ed483409.jpg)
![IHC of human lung: Using Anti-CEACAM6 [1H7-4B] (10 μg/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/796e18a7-c8c9-42df-a33e-1caf4a6a3b47.jpg)
![Immunoprecipitation of human granulocyte lysate: Utilizing 10 μg/ml Anti-CEACAM6 [1H7-4B]. Detection of the sample using 5 μg/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9d231605-4106-4c19-bab7-fb38539a7d5d.jpg)
![Western blot analysis of human granulocyte lysate: Detection using 10 μg/ml Anti-CEACAM6 [1H7-4B] followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/6fa48bbf-ecd6-4038-83c0-32925fba9d9c.jpg)
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/dd894089-cc88-43d6-ac08-3a7adf8e8fa6.jpg)
![Flow cytometry: Binding of 10 μg/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/379cbb01-0284-40b9-a6a6-f7f4ed483409.jpg)
![IHC of human lung: Using Anti-CEACAM6 [1H7-4B] (10 μg/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/796e18a7-c8c9-42df-a33e-1caf4a6a3b47.jpg)
![Immunoprecipitation of human granulocyte lysate: Utilizing 10 μg/ml Anti-CEACAM6 [1H7-4B]. Detection of the sample using 5 μg/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9d231605-4106-4c19-bab7-fb38539a7d5d.jpg)
![Western blot analysis of human granulocyte lysate: Detection using 10 μg/ml Anti-CEACAM6 [1H7-4B] followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/6fa48bbf-ecd6-4038-83c0-32925fba9d9c.jpg)
![Image thumbnail for Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/dd894089-cc88-43d6-ac08-3a7adf8e8fa6.jpg)
![Image thumbnail for Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/379cbb01-0284-40b9-a6a6-f7f4ed483409.jpg)
![Image thumbnail for Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/796e18a7-c8c9-42df-a33e-1caf4a6a3b47.jpg)
![Image thumbnail for Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9d231605-4106-4c19-bab7-fb38539a7d5d.jpg)
![Image thumbnail for Anti-CEACAM6 (CD66c) [1H7-4B] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/6fa48bbf-ecd6-4038-83c0-32925fba9d9c.jpg)
| Catalogue Number | 153324 |
| Applications | ELISA FACS IHC WB |
| Antigen/Gene or Protein Targets | CEACAM6, CD66c |
| Synonyms | CD66c |
| Reactivity | Human |
| Relevance | CEACAM6 is a glycosyl phosphatidyl inositol (GPI) linked glycoprotein. CEACAM6 expression is upregulated in many adenocarcinomas and leukemias. CEACAM6 is expressed in epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes. |
| Host | Mouse |
| Immunogen | Recombinant human CEACAM6 extracellular domain. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 90 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Strain | Balb/c |
| Notes | Clone 1H7-4B does not cross-react with human CEACAM1/3/4/5/7/8, rat CECAM1, or mouse CEACAM1/2. |
| Research Area | Immunology |
References: 6 entries
Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Europe PMC ID: 24743304
Muturi et al. 2013. PLoS One. 8(9):e74654. PMID: 24040308.
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Europe PMC ID: 24040308
Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int
Europe PMC ID: 23941132
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References: 6 entries
Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Muturi et al. 2013. PLoS One. 8(9):e74654. PMID: 24040308.
Tumor and endothelial cell-derived microvesicles carry distinct CEACAMs and influence T-cell behavior.
Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int
Add a reference
