Anti-CD3 [T1P1D4*C6]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Flow cytometric analysis of CD3gd+ cell distribution in Rainbow Trout spleen tissue using Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/92791331-4fe7-4f40-a2bb-016abeb4143e.png)
![Western blot using Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/55f7a8e0-c150-4ecd-a91f-5497c6a0ec8a.png)
![Immunohistochemistry photomicrographs showing positive staining of Anti-CD3 [T1P1D4*C6] in PKD infected fish kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9e24690f-3ad2-4d62-8c05-59a307e77515.png)
![Immunohistochemistry image showing positive membranous staining of Anti-CD3 [T1P1D4*C6] in vaccinated rainbow trout head kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/898ff216-616b-4cb9-90a9-0e3aa61962e5.png)
![Flow cytometric analysis of CD3gd+ cell distribution in Rainbow Trout spleen tissue using Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/92791331-4fe7-4f40-a2bb-016abeb4143e.png)
![Western blot using Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/55f7a8e0-c150-4ecd-a91f-5497c6a0ec8a.png)
![Immunohistochemistry photomicrographs showing positive staining of Anti-CD3 [T1P1D4*C6] in PKD infected fish kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9e24690f-3ad2-4d62-8c05-59a307e77515.png)
![Immunohistochemistry image showing positive membranous staining of Anti-CD3 [T1P1D4*C6] in vaccinated rainbow trout head kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/898ff216-616b-4cb9-90a9-0e3aa61962e5.png)
![Image thumbnail for Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/92791331-4fe7-4f40-a2bb-016abeb4143e.png)
![Image thumbnail for Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/55f7a8e0-c150-4ecd-a91f-5497c6a0ec8a.png)
![Image thumbnail for Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9e24690f-3ad2-4d62-8c05-59a307e77515.png)
![Image thumbnail for Anti-CD3 [T1P1D4*C6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/898ff216-616b-4cb9-90a9-0e3aa61962e5.png)
| Catalogue Number | 153303 |
| Applications | ELISA FACS IHC WB |
| Antigen/Gene or Protein Targets | T-cell surface glycoprotein CD3 gammadelta-A chain |
| Synonyms | CD3, CD3gd, cd3g/d, cd3-gamma |
| Reactivity | Salmon and Rainbow Trout |
| Relevance |
Monoclonal antibody specific for CD3 in Atlantic salmon and rainbow trout. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. Anti-CD3 [T1P1D4*C6] is specific for CD3 in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY |
| Immunogen UniProt ID | A3RK68 |
| Positive Control | Western Blot, Spleen lysates; IHC, Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout; FACS, Spleen cells, peripheral blood lymphocyte (PBL) |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-CD3 [T1P1D4*C6] was used to help suggest that following peritoneal stimulation the visceral adipose mass in fish likely plays an important role in vaccine antigen uptake and presentation by APCs, as well as subsequent T-cell activation and differentiation. Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY, 91% sequence identity with large yellow croaker (Larimichthys crocea), Sablefish (Anoplopoma fimbria), 82% sequence identity with fugu (Takifugu rubripes), snappers (Lutjanus sanguineus). Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC 1/3 dilution (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0); Flow cytomtery 1/4-1/8 dilution (cells were fixed and permeabilized by adding 100 µl of BD Cytofix/Cytoperm to 1 × 105 lymphocytes for 15 min) Concentration 1mg/ml as standard |
| Research Area | Aquaculture, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology |
References: 2 entries
Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214.
Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination.
Europe PMC ID: 30731214
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References: 2 entries
Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214.
Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination.
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