Cancer Research Technology
Log in Register
Menu

Anti-CD3 [T1P1D4*C6]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 153303
Applications ELISA FACS IHC WB
Antigen/Gene or Protein Targets T-cell surface glycoprotein CD3 gammadelta-A chain
Synonyms CD3, CD3gd, cd3g/d, cd3-gamma
Reactivity Salmon and Rainbow Trout
Relevance Anti-CD3 [T1P1D4*C6] is specific for CD3 in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY
Positive Control Western Blot, Spleen lysates; IHC, Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout; FACS, Spleen cells, peripheral blood lymphocyte (PBL)
Subclass IgG1 kappa
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY, 91% sequence identity with large yellow croaker (Larimichthys crocea), Sablefish (Anoplopoma fimbria), 82% sequence identity with fugu (Takifugu rubripes), snappers (Lutjanus sanguineus).

Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC 1/3 dilution (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0); Flow cytomtery 1/4-1/8 dilution (cells were fixed and permeabilized by adding 100 µl of BD Cytofix/Cytoperm to 1 × 105 lymphocytes for 15 min)
Research Area Aquaculture, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References

There are 2 reference entries for this reagent.

View All References

References: 2 entries

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214.

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination.

Europe PMC ID: 30731214


Add a reference

References: 2 entries

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214.

Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination.


Add a reference