![Flow cytometric analysis of CD3gd+ cell distribution in Rainbow Trout spleen tissue using Anti-CD3 [T1P1D4*C6]](https://cancertools.org/wp-content/uploads/92791331-4fe7-4f40-a2bb-016abeb4143e.png "92791331-4fe7-4f40-a2bb-016abeb4143e.png")
Cat. #153303
Anti-CD3 [T1P1D4*C6]
Cat. #: 153303
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-5 days
Target: T-cell surface glycoprotein CD3 gammadelta-A chain
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; WB
Reactivity: Salmon ; Rainbow Trout
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ayham Alnabulsi
Institute: Vertebrate Antibodies Limited
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-CD3 [T1P1D4*C6]
- Alternate name: CD3, CD3gd, cd3g/d, cd3-gamma
- Clone: T1P1D4*C6
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Salmon ; Rainbow Trout
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; WB
- Description: Monoclonal antibody specific for CD3 in Atlantic salmon and rainbow trout. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3? and 2X CD3?), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. Anti-CD3 [T1P1D4*C6] is specific for CD3 in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).
- Immunogen: Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY
- Immunogen uniprot id: A3RK68
- Isotype: IgG1 kappa
- Myeloma used: P3X63Ag8.653
- Recommended controls: Western Blot, Spleen lysates; IHC, Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout; FACS, Spleen cells, peripheral blood lymphocyte (PBL)
Target Details
- Target: T-cell surface glycoprotein CD3 gammadelta-A chain
- Tissue cell line specificity: Western Blot, Spleen lysates; IHC, Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout; FACS, Spleen cells, peripheral blood lymphocyte (PBL)
- Target background: Monoclonal antibody specific for CD3 in Atlantic salmon and rainbow trout.
Background and Research Application
The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3d and 2X CD3
Applications
- Application: ELISA ; FACS ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214.
- Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination.
