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Catalogue Number
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153284 |
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Applications
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ELISA
IHC
WB
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Antigen/Gene or Protein Targets
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Programmed Cell Death 1 Ligand 2 (PDL2-Soluble form)
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Synonyms
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B7 dendritic cell molecule, B7-DC, B7DC, bA574F11.2, Btdc, Butyrophilin B7 DC, Butyrophilin B7-DC, Butyrophilin B7DC, CD 273, CD273, CD273 antigen, MGC142238, MGC142240, PD 1 ligand 2, PD L2, PD-1 ligand 2, PD-L2, PD1 ligand 2, PD1L2_HUMAN, PDCD 1 ligand 2, PDCD1 ligand 2
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Reactivity
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Human
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Relevance
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Recombinant antibody of PDL2 which can aid understanding of programmed death ligand 2, in the secreted form.
Background and Research Application
PDL2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.). PDL2 is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response.PD-L2 is one of two known ligands for Programmed cell death protein 1 (PD-1).
This is a recombinant version of the anti-PDL2 monoclonal antibody . PD-1, is a key immune checkpoint. Currently several anti-PD-1 antibodies or blockers are in cancer clinical trials and showing promising outcomes. This antibody will allow scientists examine, for the first time, the clinical significance of PDL2-secreted form.
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Host
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Mouse
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Immunogen
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Ovalbumin-conjugated synthetic peptide KAVFFKRHN
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Subclass
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Not Known
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Formulation
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PBS
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Concentration
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1mgml-1
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Positive Control
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IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray
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Research Area
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Immunology
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Immunogen UniProt ID
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Q9BQ51
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Notes
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Production Details
Purified using multi-step affinity chromatography with protein A.
Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
For IHC perform antigen retrieval step: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0
Recombinant monoclonal antibody produced from the original monoclonal cell line. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.
Five products based on this clone:
- Anti-PDL2 [Z64P2D3*H4], Human IgG1, Kappa
- Anti-PDL2 [Z64P2D3*H4], Human IgG1, Kappa, engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors
- Anti-PDL2 [Z64P2D3*H4], Human IgG4-S228P, Kappa
- Anti-PDL2 [Z64P2D3*H4], Mouse IgG1, Kappa
- Anti-PDL2 [Z64P2D3*H4], Rabbit IgG, Kappa
Concentration
1mg/ml as standard
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![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human testis seminoma tissue sections using anti-PDL2 (soluble form) [Z64P2D3*H4]. Cytoplasmic staining was observed.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/1fd95695-faff-4626-a2cf-2a4d07b8b7d5.jpg)
