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Anti-5-Hydroxymethylcytosine (5hmc), Recombinant [AB3/63.3]

Info

Catalogue Number 153274
Applications IF IP DB
Antigen/Gene or Protein Targets 5-Hydroxymethylcytosine (5hmC)
Reactivity All
Relevance The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Host Rat
Immunogen 5-Hydroxymethylcytosine conjugated to BSA
Subclass IgG2a kappa
Formulation PBS
Concentration 1mgml-1
Positive Control Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Research Area Epigenetics & Nuclear Signalling
Notes Recombinant monoclonal antibody produced from the original monoclonal cell line. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Three products based on this clone:
- Anti-5-Hydroxymethylcytosine [AB3/63.3], Rat IgG2a, Kappa
- Anti-5-Hydroxymethylcytosine [AB3/63.3], Rat IgG2a, Kappa, engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors
- Anti-5-Hydroxymethylcytosine [AB3/63.3], Rabbit IgG, Kappa

References

There are 1 reference entries for this reagent.

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References: 1 entry

Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.


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References: 1 entry

Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.


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