Cat. #153274
Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] rAb
Cat. #: 153274
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: 5-Hydroxymethylcytosine (5hmC)
Class: Recombinant
Application: IF ; IP ; DB
Reactivity: All
Host: Rat
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Wolf Reik
Institute: Absolute Antibody ; Babraham Institute
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] rAb
- Clone: AB3/63.3
- Tool sub type: Primary antibody
- Class: Recombinant
- Purpose: Marker
- Conjugation: Unconjugated
- Reactivity: All
- Host: Rat
- Application: IF ; IP ; DB
- Description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact...
- Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
- Isotype: IgG2a kappa
- Recommended controls: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Target Details
- Target: 5-Hydroxymethylcytosine (5hmC)
- Tissue cell line specificity: Mouse
- Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact...
Applications
- Application: IF ; IP ; DB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Shipping conditions: Shipping at 4° C
References
- Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
