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Anti-RNA polII, Recombinant [CTD 4H8]

Info

Catalogue Number 153268
Applications ChIP ELISA FACS IHC IF IP WB ChIP-seq
Antigen/Gene or Protein Targets RNA Polymerase II
Reactivity Human and Saccharomyces cerevisiae
Relevance RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Host Mouse
Immunogen Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Subclass IgG1
Formulation PBS
Concentration 1mgml-1
Positive Control HeLa cell line.
Research Area Epigenetics & Nuclear Signalling
Notes Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein.
Recombinant monoclonal antibody produced from the original monoclonal cell line. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Five products based on this clone:
- Anti-RNA polymerase II [CTD 4H8], Mouse IgG1, Kappa
- Anti-RNA polymerase II [CTD 4H8], Rabbit IgG, Kappa
- Anti-RNA polymerase II [CTD 4H8], Human IgG1, Kappa
- Anti-RNA polymerase II [CTD 4H8], Human IgG1, Kappa, engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors
- Anti-RNA polymerase II [CTD 4H8], Human IgG4-S228P, Kappa

References

There are 1 reference entries for this reagent.

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References: 1 entry

Original hybridoma first published in: Kristjuhan et al. 2002. Mol Cell. 10(4):925-33. PMID: 12419235.


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References: 1 entry

Original hybridoma first published in: Kristjuhan et al. 2002. Mol Cell. 10(4):925-33. PMID: 12419235.


Add a reference