Cat. #153268
Anti-RNA polII [CTD 4H8] rAb
Cat. #: 153268
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: RNA Polymerase II
Class: Recombinant
Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
Reactivity: Human ; Saccharomyces cerevisiae
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jesper Svejstrup
Institute: Absolute Antibody ; Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-RNA polII [CTD 4H8] rAb
- Clone: CTD 4H8
- Tool sub type: Primary antibody
- Class: Recombinant
- Conjugation: Unconjugated
- Reactivity: Human ; Saccharomyces cerevisiae
- Host: Mouse
- Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
- Description: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
- Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
- Isotype: IgG1
- Recommended controls: HeLa cell line.
Target Details
- Target: RNA Polymerase II
- Tissue cell line specificity: HeLa cell line.
- Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Applications
- Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS
- Shipping conditions: Shipping at 4° C
References
- Original hybridoma first published in: Kristjuhan et al. 2002. Mol Cell. 10(4):925-33. PMID: 12419235.
