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Polyclonal Antibodies

Anti-HPV16E2, Polyclonal [HPV16E2]

Invented at A*STAR Accelerate Technologies Pte Ltd
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Figure. E2 induces a DNA damage signal in A549 cells arrested in prophase. A. Western blot analyses of lysates from A549 cells expressing GFP-16E2, GFP-18E2, HPV18E2 transactivation domain (GFP-18E2TAD) or the DNA binding domain (GFP-18E2DBD), probed with antibodies against GFP, γH2AX, Chk2T68 and ATMS1981. B. Confocal microscopy of E2-expressing A549 cells double labeled with anti-γH2AX (light blue) and anti-Chk2T68 (red). White arrows: co-localization of the DDR markers, red arrows: DDR marker partial co-localization with 16E2 in infected nuclei. C. As in (B) except the cells were co-labeled with anti-H3p (red) and anti-γH2AX (light blue). Inset show higher magnification of double stained infected nuclei with distinct punctate signals for the H3p and γH2AX markers D. Expression of H3p (red) and γH2AX (green) in A549 cells treated with increasing doses of etoposide. Scale bar = 10μm.
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Catalogue Number 153202
Applications IHC WB
Antigen/Gene or Protein Targets HPV16E2
Synonyms Human papillomavirus type 16; Regulatory protein E2
Reactivity Human
Relevance Cervical intraepithelial neoplasia (CIN) is caused by human papillomavirus (HPV) infection and is the precursor to cervical carcinoma. The completion of the HPV productive life cycle depends on the expression of viral proteins. Initiation of the viral productive replication requires expression of the E2 viral protein that cooperates with the E1 viral DNA helicase. A novel and important role for the HPV16-E2 protein in controlling host cell cycle during malignant transformation was recently revealed. Xells expressing HPV16-E2 in vitro were arrested in prophase alongside activation of a sustained DDR signal. In vivo, HPV16-E2 protein is present in cells that express both mitotic and DDR signals specifically in CIN3 lesions, immediate precursors of cancer, suggesting that E2 may be one of the drivers of genomic instability and carcinogenesis in vivo.
Host Rabbit
Immunogen GST-HPV16 E2 (209–365)
Formulation Whole serum
Research Area Cancer, Cell Cycle, Virology
Immunogen UniProt ID TBC

References

There are 4 reference entries for this reagent.

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References: 4 entries

Xue et al. 2015. Oncotarget. 6(33):34979-91. PMID: 26474276.

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HPV16-E2 induces prophase arrest and activates the cellular DNA damage response in vitro and in precursor lesions of cervical carcinoma.

Europe PMC ID: 26474276

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Xue et al. 2010. Cancer Res. 70(13):5316-25. PMID: 20530671.

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HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma.

Europe PMC ID: 20530671

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References: 4 entries

Xue et al. 2015. Oncotarget. 6(33):34979-91. PMID: 26474276.

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HPV16-E2 induces prophase arrest and activates the cellular DNA damage response in vitro and in precursor lesions of cervical carcinoma.

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Xue et al. 2010. Cancer Res. 70(13):5316-25. PMID: 20530671.

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HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma.

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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