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HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line

Invented by Prof Francis Barr at University of Oxford

Info

Catalogue Number 152987
Antigen/Gene or Protein Targets Histone H2B, Alpha Tubulin
Parental Line HeLa
Host Human
Tissue Cervix
Model Reporter
Relevance The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP. Both constructs were transfected into human HeLa cells to generate a stable line constitutively expressing H2B-mCherry and EGFP-Alpha Tubulin. The mCherry-Histone H2B fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
Research Area Cell Cycle, Cell Type or Organelle Marker, Cell Structure and Motility, DNA Damage and Repair, Genetic Studies Tools
Recommended Growing Conditions DMEM, 10% FBS, 5% CO2, 37oC. Antibiotic selection for GFP and mCherry expression: 1μg/mL Puromycin, 4μg/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended.

References

There are 6 reference entries for this reagent.

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References: 6 entries

Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.

Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.

Europe PMC ID: 22965910

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Europe PMC ID: 21187329

Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.

Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission.

Europe PMC ID: 21079244


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References: 6 entries

Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.

Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.

Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission.


Add a reference