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HeLa EGFP-Histone H2B Cell Line

Invented by Prof Francis Barr at University of Oxford

Info

Catalogue Number 152940
Parental Line HeLa
Synonyms Histone H2B
Host Human
Tissue Cervix
Model Reporter
Relevance The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
Research Area Cell Cycle, Cell Type or Organelle Marker, Cell Structure and Motility, DNA Damage and Repair, Genetic Studies Tools
Recommended Growing Conditions DMEM, 10% FBS, 5% CO2, 37oC. Antibiotic resistance for selection of GFP positive cells: 4μg/mL Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended.

References

There are 2 reference entries for this reagent.

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References: 2 entries

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Europe PMC ID: 21187329


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References: 2 entries

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.


Add a reference