HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line
Invented by Prof Francis Barr at University of Liverpool
HeLa EGFP-Histone H2B Cell Line
Invented by Prof Francis Barr from University of Oxford
Invented at University of Liverpool
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152940 |
| Parental Line | HeLa |
| Synonyms | Histone H2B |
| Host | Human |
| Tissue | Cervix |
| Model | Reporter |
| Relevance | The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin. |
| Research Area | Cell Cycle, Cell Type or Organelle Marker, Cell Structure and Motility, DNA Damage and Repair, Genetic Studies Tools |
| Recommended Growing Conditions | DMEM, 10% FBS, 5% CO2, 37oC. Antibiotic resistance for selection of GFP positive cells: 4μg/mL Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended. |
References: 2 entries
Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Europe PMC ID: 21187329
Add a reference
References: 2 entries
Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Add a reference
