D34 Cell Line
Invented at Cancer Research UK Glasgow: The Beatson Institute
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152857 |
| Host | Human |
| Tissue | Tongue |
| Disease Keywords | Dysplasia, Leukoplakia |
| Model | Tumour line |
| Relevance | D34 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D34 was categorised as having a moderate dysplasia pathology. |
| Production Details | Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders, in 10H medium. The 3T3 feeder layer was removed by treatment with 0.02% EDTA prior to RNA and protein extraction. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Recommended Growing Conditions | As per Cancer Res. 1997 Sep 15;57(18):3886-9. All cells were maintained on irradiated 3T3 feeders, in 10H medium (DMEM plus 10% FCS without added growth factors except hydrocortisone). |
| Cellosaurus ID | CVCL_HF98 |
References: 2 entries
McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.
Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.
Europe PMC ID: 12183435
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References: 2 entries
McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.
Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.
Add a reference
