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D38 Cell Line

Info

Catalogue Number 152856
Host Human
Tissue Tongue
Disease Keywords Dysplasia, Leukoplakia
Model Tumour line
Relevance D38 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D38 was categorised as having a mild dysplasia pathology.
Production Details Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders, in 10H medium. The 3T3 feeder layer was removed by treatment with 0.02% EDTA prior to RNA and protein extraction.
Conditional No
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions As per Cancer Res. 1997 Sep 15;57(18):3886-9. All cells were maintained on irradiated 3T3 feeders, in 10H medium (DMEM plus 10% FCS without added growth factors except hydrocortisone).
Cellosaurus ID CVCL_HF99

References

There are 2 reference entries for this reagent.

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References: 2 entries

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.

Europe PMC ID: 12183435


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References: 2 entries

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.


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