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Catalogue Number 152814
Applications ELISA FACS IHC IP Fn WB
Antigen/Gene or Protein Targets Mucin1 (MUC1)
Synonyms ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
Reactivity Human
Relevance Mucin-1 (MUC1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MUC1 is also localised on the surface of the human milk fat globule. MUC1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. It is upregulated in the lactating breast and in carcinomas.
Host Mouse
Immunogen Milk fat globule followed epithelial cells cultured from milk
Subclass IgG1 lambda
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS - see Notes
Strain Balb/c
Notes Clone also called "3.14.A3" & "F314AE11".

Clone HMFG2 requires very specific culture conditions. Under stress the hybridoma switches it's light chain from lambda to kappa : Recover a vial into a T25 flask into RPMI containing 10% serum. Stand the flask upright and after a few hours (about 4-5) if the cells look large, round and healthy lay it on its side adding more medium if necessary. The cells should be monitored every day and split 1:3 before they too dense. The cells should be relatively large, round and shiny. When they are under stress they quickly become smaller and slightly granular – if this happens it is really too late to do anything and another vial should be recovered. When the cells have been moved up to large flask T180 the same regime should be followed but I used to split 1 flask 1:3 and leave the other 2 flask an extra day or two before harvesting the medium.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction

References: 14 entries

Kaur et al. 2014. PLoS One. 9(3):e92742. PMID: 24671186.

IHC WB

Altered expression of transmembrane mucins, MUC1 and MUC4, in bladder cancer: pathological implications in diagnosis.

Europe PMC ID: 24671186

Schettini et al. 2012. Cancer Immunol Immunother. 61(11):2055-65. PMID: 22543528.

Fn

Intratumoral delivery of CpG-conjugated anti-MUC1 antibody enhances NK cell anti-tumor activity.

Europe PMC ID: 22543528

Wilkie et al. 2008. J Immunol. 180(7):4901-9. PMID: 18354214.

Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.

Europe PMC ID: 18354214

Sakurai et al. 2007. Eur J Histochem. 51(2):95-102. PMID: 17664159.

IHC

Differential expression of the glycosylated forms of MUC1 during lung development.

Europe PMC ID: 17664159

Schroeder et al. 2001. J Biol Chem. 276(16):13057-64. PMID: 11278868.

WB IP

Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland.

Europe PMC ID: 11278868

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Europe PMC ID: 7505698

Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

Europe PMC ID: 7309278


Add a reference

References: 14 entries

Kaur et al. 2014. PLoS One. 9(3):e92742. PMID: 24671186.

IHC WB

Altered expression of transmembrane mucins, MUC1 and MUC4, in bladder cancer: pathological implications in diagnosis.

Europe PMC ID: 24671186

Schettini et al. 2012. Cancer Immunol Immunother. 61(11):2055-65. PMID: 22543528.

Fn

Intratumoral delivery of CpG-conjugated anti-MUC1 antibody enhances NK cell anti-tumor activity.

Europe PMC ID: 22543528

Wilkie et al. 2008. J Immunol. 180(7):4901-9. PMID: 18354214.

Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.

Europe PMC ID: 18354214

Sakurai et al. 2007. Eur J Histochem. 51(2):95-102. PMID: 17664159.

IHC

Differential expression of the glycosylated forms of MUC1 during lung development.

Europe PMC ID: 17664159

Schroeder et al. 2001. J Biol Chem. 276(16):13057-64. PMID: 11278868.

WB IP

Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland.

Europe PMC ID: 11278868

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Europe PMC ID: 7505698

Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

Europe PMC ID: 7309278


Add a reference

References: 14 entries

Kaur et al. 2014. PLoS One. 9(3):e92742. PMID: 24671186.

IHC WB

Altered expression of transmembrane mucins, MUC1 and MUC4, in bladder cancer: pathological implications in diagnosis.

Schettini et al. 2012. Cancer Immunol Immunother. 61(11):2055-65. PMID: 22543528.

Fn

Intratumoral delivery of CpG-conjugated anti-MUC1 antibody enhances NK cell anti-tumor activity.

Wilkie et al. 2008. J Immunol. 180(7):4901-9. PMID: 18354214.

Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.

Sakurai et al. 2007. Eur J Histochem. 51(2):95-102. PMID: 17664159.

IHC

Differential expression of the glycosylated forms of MUC1 during lung development.

Schroeder et al. 2001. J Biol Chem. 276(16):13057-64. PMID: 11278868.

WB IP

Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland.

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.


Add a reference


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