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Monoclonal Antibodies

Anti-MUC1 [HMFG2]

Invented by Prof Joyce Taylor-Papadimitriou from King's College London , Prof Joy Burchell from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Expression of MUC1 in non-neoplastic bladder urothelium, non-invasive papillary urothelial carcinoma, urothelial carcinoma in situ and invasive high grade urothelial carcinoma. Bladder tumor tissue sections or spots were probed with anti-MUC1 mAb (HMFG2) after non-specific blocking with horse serum. All sections were examined under microscope and the immunoreactivity was evaluated by reddish brown staining. Representative photomicrographs are shown for MUC1 stained non-neoplastic bladder urothelium (A&B), low-grade papillary carcinoma (C), bladder carcinoma in situ (D), and high grade invasive cancer (E&F). In normal bladder urothelium, MUC1 expression was restricted to umbrella cells (shown by arrowhead in panel A and the magnified inset) in majority of samples. In some samples, a sheath of MUC1 mucin was observed over urothelium (arrow in 1B). In low-grade papillary carcinoma, intense staining of MUC1 was observed on the luminal surface of the urothelium and lower intensity of staining was observed in the other layers of urothelium (C). In urothelial carcinoma in situ, MUC1 expression was observed in all the layers of urothelium, however, staining was comparatively stronger in cells closer to the luminal border (D). In invasive high grade urothelial carcinoma, MUC1 staining was observed in cells invading singly (E) or as small groups within the lamina propria (F). Taken from PLoS One. 2014 Mar 26;9(3):e92742. doi: 10.1371/journal.pone.0092742. eCollection 2014.
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Catalogue Number 152814
Applications ELISA FACS IHC IP Fn WB
Antigen/Gene or Protein Targets Mucin1 (MUC1)
Synonyms ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
Reactivity Human
Relevance Monoclonal antibody which detects several glycoforms of MUC1, a marker of breast cancer.

Background and Research Application
Mucin-1 (MUC1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MUC1 is also localised on the surface of the human milk fat globule. MUC1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development and can be used to monitor response to breast cancer treatment and disease recurrence. Lower levels over time may be indicative of a positive response to treatment. It is upregulated in the lactating breast and in carcinomas. MUC1 also is involved in tumour progression and transcription through regulation of p53.
Anti-MUC1 can react with unglycosylated MUC1, and several glycoforms.
Host Mouse
Immunogen Milk fat globule followed epithelial cells cultured from milk
Immunogen UniProt ID P15941
Subclass IgG1 lambda
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS - see Notes
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody immunostains human breast cancer tissues.

Clone also called "3.14.A3" & "F314AE11".

Clone HMFG2 requires very specific culture conditions. Under stress the hybridoma switches it's light chain from lambda to kappa : Recover a vial into a T25 flask into RPMI containing 10% serum. Stand the flask upright and after a few hours (about 4-5) if the cells look large, round and healthy lay it on its side adding more medium if necessary. The cells should be monitored every day and split 1:3 before they too dense. The cells should be relatively large, round and shiny. When they are under stress they quickly become smaller and slightly granular – if this happens it is really too late to do anything and another vial should be recovered. When the cells have been moved up to large flask T180 the same regime should be followed but I used to split 1 flask 1:3 and leave the other 2 flask an extra day or two before harvesting the medium.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction

References

There are 19 reference entries for this reagent.

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References: 19 entries

Mei et al. 2020. Cancer Med. 9(2):640-652. PMID: 31800160.

View  

Yoshimura et al. 2019. Sci Rep. 9(1):16641. PMID: 31719620.

View  

Guedan et al. 2019. Mol Ther Methods Clin Dev. 12:145-156. PMID: 30666307.

View  

Balmaña et al. 2018. Molecules. 23(11):. PMID: 30380716.

View  

Nair et al. 2018. Indian J Dermatol. 63(5):440-442. PMID: 30210176.

View  

Kaur et al. 2014. PLoS One. 9(3):e92742. PMID: 24671186.

IHC WB

View  

Altered expression of transmembrane mucins, MUC1 and MUC4, in bladder cancer: pathological implications in diagnosis.

Europe PMC ID: 24671186

View  

Schettini et al. 2012. Cancer Immunol Immunother. 61(11):2055-65. PMID: 22543528.

Fn

View  

Intratumoral delivery of CpG-conjugated anti-MUC1 antibody enhances NK cell anti-tumor activity.

Europe PMC ID: 22543528

View  

Wilkie et al. 2008. J Immunol. 180(7):4901-9. PMID: 18354214.

View  

Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.

Europe PMC ID: 18354214

View  

Sakurai et al. 2007. Eur J Histochem. 51(2):95-102. PMID: 17664159.

IHC

View  

Differential expression of the glycosylated forms of MUC1 during lung development.

Europe PMC ID: 17664159

View  

Schroeder et al. 2001. J Biol Chem. 276(16):13057-64. PMID: 11278868.

WB IP

View  

Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland.

Europe PMC ID: 11278868

View  

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

View  

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Europe PMC ID: 7505698

View  

Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

View  

Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

Europe PMC ID: 7309278

View  

Add a reference

References: 19 entries

Mei et al. 2020. Cancer Med. 9(2):640-652. PMID: 31800160.

View  

Yoshimura et al. 2019. Sci Rep. 9(1):16641. PMID: 31719620.

View  

Guedan et al. 2019. Mol Ther Methods Clin Dev. 12:145-156. PMID: 30666307.

View  

Balmaña et al. 2018. Molecules. 23(11):. PMID: 30380716.

View  

Nair et al. 2018. Indian J Dermatol. 63(5):440-442. PMID: 30210176.

View  

Kaur et al. 2014. PLoS One. 9(3):e92742. PMID: 24671186.

IHC WB

View  

Altered expression of transmembrane mucins, MUC1 and MUC4, in bladder cancer: pathological implications in diagnosis.

View  

Schettini et al. 2012. Cancer Immunol Immunother. 61(11):2055-65. PMID: 22543528.

Fn

View  

Intratumoral delivery of CpG-conjugated anti-MUC1 antibody enhances NK cell anti-tumor activity.

View  

Wilkie et al. 2008. J Immunol. 180(7):4901-9. PMID: 18354214.

View  

Retargeting of human T cells to tumor-associated MUC1: the evolution of a chimeric antigen receptor.

View  

Sakurai et al. 2007. Eur J Histochem. 51(2):95-102. PMID: 17664159.

IHC

View  

Differential expression of the glycosylated forms of MUC1 during lung development.

View  

Schroeder et al. 2001. J Biol Chem. 276(16):13057-64. PMID: 11278868.

WB IP

View  

Transgenic MUC1 interacts with epidermal growth factor receptor and correlates with mitogen-activated protein kinase activation in the mouse mammary gland.

View  

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

View  

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

View  

Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

View  

Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

View  

Add a reference

Inventor Information