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Anti-BORIS [20B11]
RECENTLY UPDATED

Invented by Prof Elena Klenova at University of Essex

Info

Catalogue Number 152812
Applications ChIP ELISA IHC IF IP WB
Antigen/Gene or Protein Targets BORIS
Synonyms Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor (zinc finger protein) like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, Ctcfl, CTCFL_HUMAN, dJ579F20.2, HMG 1L1, HMGB1L1, MGC163358, MGC169105, MGC169106
Reactivity Human
Relevance Reagent for research, diagnostic tool. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that d BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50).
Host Mouse
Immunogen Synthetic peptide within the BORIS C-terminal domain (aa 614-648) (CG)GEMFPVACRETTARVKEE (NB - the first two aa do not belong to BORIS)
Positive Control MCF7 cell lysates
Subclass IgG3 kappa
Molecular Weight (kDa) 83
Recommended Growing Conditions Vials with hybridoma cell from the liquid nitrogen storage should be rapidly thawed. After thawing, the cell suspension needs to be added slowly using a sterile pipette into 10ml of warmed HM20 medium, the suspended cells centrifuged at 1200 rpm for 10 min and the supernatant discarded. The cell pellet should be gently re-suspended in 9ml of HM20-10% HCF (Hybridoma Cloning Factor, BioVeris Corporation) and transferred into a small flask (T25). Cells should be incubated at 37°C and 10% CO2 and passaged when they were 90% confluent. Cells should be successively grown in the medium with gradually decreasing amounts of HCF from 10% to 5% to 2.5% and finally without any HCF. Depending upon the confluency of the culture, cells should be transferred from the small flask to a medium sized flask (T75) during one of these stages.
Research Area
RECENTLY UPDATED
Cancer, Epigenetics & Nuclear Signalling

References

There are 1 reference entries for this reagent.

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References: 1 entry
RECENTLY UPDATED

Debruyne et al. 2019. Nature. 572(7771):676-680. PMID: 31391581.


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References: 1 entry
RECENTLY UPDATED

Debruyne et al. 2019. Nature. 572(7771):676-680. PMID: 31391581.


Add a reference