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Anti-BORIS [4A7]
RECENTLY UPDATED

Invented by Prof Elena Klenova at University of Essex

Info

Catalogue Number 151848
Applications ChIP ELISA IF IP WB
Antigen/Gene or Protein Targets BORIS
Synonyms Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor (zinc finger protein) like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, Ctcfl, CTCFL_HUMAN, dJ579F20.2, HMG 1L1, HMGB1L1, MGC163358, MGC169105, MGC169106
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody to understand the role of BORIS protein in prostate cancers.

Background and Research Application
BORIS protein is a transcriptional repressor encoded by CTCFL gene, and is normally present at high levels in testis.
BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50). BORIS protein is detected in all prostate cell lines and prostate tumours, but it is absent in benign prostatic hyperplasia tissues. Increased BORIS protein levels correlate with higher Gleason scores, T-stage and androgen receptor (AR) protein levels in prostate tumours.
This reagent is for research and as a diagnostic tool.
Host Mouse
Immunogen The N-terminal domain (aa 2-257) of human BORIS (CTCFL) protein, expressed in E.coli
Immunogen UniProt ID
RECENTLY UPDATED
Q8NI51
Positive Control Testis
Subclass IgM kappa
Molecular Weight (kDa) 83
Recommended Growing Conditions HM20-10% HCF (Further details in remarks)
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Detection of BORIS in prostate tumours suggests potential applications of BORIS as a biomarker for prostate cancer diagnosis, as an immunotherapy target and, potentially, a prognostic marker of more aggressive prostate cancer.

Protein L beads should be used for ChIP and IP because this antibody is IgM kappa

Staining Pattern: May be nuclear, cytoplasmic or both (depending on cell type)

Concentration
1mg/ml as standard
Research Area Cancer, Epigenetics & Nuclear Signalling

References

There are 2 reference entries for this reagent.

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References: 2 entries

Cheema et al. 2014. Prostate. 74(2):164-76. PMID: 24123052.

Expression of the cancer-testis antigen BORIS correlates with prostate cancer.

Europe PMC ID: 24123052


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References: 2 entries

Cheema et al. 2014. Prostate. 74(2):164-76. PMID: 24123052.

Expression of the cancer-testis antigen BORIS correlates with prostate cancer.


Add a reference