Log in Register
Menu

Human Lung Stem Cell p38alpha KO (SH3) Line

Info

Catalogue Number 152716
Antigen/Gene or Protein Targets p38alpha mitogen-activated protein (MAP) kinase (MAPK14)
Disease Keywords Lung, cancer, fibrosis
Relevance HLSC p38alpha KO express lentiviral constructs to produce stable p38alpha knockdown (>90%). p38alpha MAPK has been shown to regulate homeostasis of lung stem cells. These cells show increased proliferation and disruption of expression of both lung markers and integrins, and higher levels of stem cell marker Sox9.
Production Details p38α shRNA cloned into the PLKO.1‐TRC lentiviral vector to generate a p38α knockdown construct (SH3). Infectious virus was added to cells in the presence of 8 mg/ml polybrene and incubated for 6 h.
Conditional No
Research Area Metabolism, Stem Cell Biology
Recommended Growing Conditions Serum‐free basal stem cell culture medium containing growth factors FGF (10 ng ml-1) and EGF (20 ng ml-1) (37°C in a 7% humidified CO2 incubator)
Cellosaurus ID CVCL_AS78

References: 1 entry

Oeztuerk-Winder et al. 2012. EMBO J. 31(16):3431-41. PMID: 22828869.


Add a reference

References: 1 entry

Oeztuerk-Winder et al. 2012. EMBO J. 31(16):3431-41. PMID: 22828869.


Add a reference

References: 1 entry

Oeztuerk-Winder et al. 2012. EMBO J. 31(16):3431-41. PMID: 22828869.


Add a reference


This reagent does not have any reviews at the moment.