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Anti-5T4 [B3F1]

Info

Catalogue Number 152711
Applications ELISA FACS IF Fn WB
Antigen/Gene or Protein Targets 5T4 oncofetal glycoprotein
Synonyms Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
Reactivity Mouse
Relevance The murine 5T4 monoclonal antibody, clone B3F1, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
Host Mouse
Subclass IgG2a
Molecular Weight (kDa) 46.5
Recommended Growing Conditions DMEM supplemented with heat inactivated 10% fetal calf serum, 2 mM L-glutamine, 100 units/ml penicillin and 100 µg/ml streptomycin solution and maintained at 37°C in a humidified atmosphere of 5% CO2/95% air.
Strain C57BL/6
Notes Clone B3F1 inhibits the chemotactic migration towards CXCL12 exhibited by differentiating WT-ES cells (to a lesser extent than clone B1C3). IC50 for the maximally inhibitory mAb B3F1 was 2.2 µg±0.8.

Epitope: distal LRR domain of m5T4
Research Area Cancer, Stem Cell Biology

References: 3 entries

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.


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References: 3 entries

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.


Add a reference

References: 3 entries

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.


Add a reference


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