Cancer Research Technology
Log in Register
Menu

Anti-5T4 [B3F1]
RECENTLY UPDATED

Info

Catalogue Number 152711
Applications ELISA FACS IF Fn WB
Antigen/Gene or Protein Targets 5T4 oncofetal glycoprotein
Synonyms Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
Reactivity Mouse
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours.

Background and Research Application
The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ β-catenin signalling pathway.
Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours.
5T4 is a potential target for T cells, in antibody-directed immunotherapy.
Host Mouse
Immunogen UniProt ID
RECENTLY UPDATED
Q9Z0L0
Subclass IgG2a
Molecular Weight (kDa) 46.5
Recommended Growing Conditions DMEM supplemented with heat inactivated 10% fetal calf serum, 2 mM L-glutamine, 100 units/ml penicillin and 100 µg/ml streptomycin solution and maintained at 37°C in a humidified atmosphere of 5% CO2/95% air.
Strain C57BL/6
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
A study found that 5T4 was present in 85% of a study of 72 colorectal carcinomas and in 81% of a study of 27 gastric carcinomas.

Clone B3F1 inhibits the chemotactic migration towards CXCL12 exhibited by differentiating WT-ES cells (to a lesser extent than clone B1C3). IC50 for the maximally inhibitory mAb B3F1 was 2.2 µg±0.8.

Epitope: distal LRR domain of m5T4

Concentration
1mg/ml as standard
Research Area
RECENTLY UPDATED
Cancer, Stem Cell Biology

References

There are 7 reference entries for this reagent.

View All References

References: 7 entries
RECENTLY UPDATED

Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.

Europe PMC ID: 20376365

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.

Europe PMC ID: 14576347

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.

Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.

Europe PMC ID: 12003637


Add a reference

References: 7 entries
RECENTLY UPDATED

Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.

Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.

CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.

Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.

The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.

Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.

Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor