Cat. #152711
Anti-5T4 [B3F1]
Cat. #: 152711
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: 5T4 oncofetal glycoprotein
Class: Monoclonal
Application: ELISA ; FACS ; IF ; Fn ; WB
Reactivity: Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Stern
Institute: Cancer Research UK, Manchester Institute
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-5T4 [B3F1]
- Alternate name: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
- Clone: B3F1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 46.5 kDa
- Strain: C57BL/6
- Reactivity: Mouse
- Host: Mouse
- Application: ELISA ; FACS ; IF ; Fn ; WB
- Description: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
- Immunogen: Q9Z0L0
- Immunogen uniprot id: Q9Z0L0
- Isotype: IgG2a
Target Details
- Target: 5T4 oncofetal glycoprotein
- Molecular weight: 46.5 kDa
- Target background: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
Applications
- Application: ELISA ; FACS ; IF ; Fn ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-5T4 [B1C3] ; Anti-5T4 [B5C9] ; Anti-5T4 [B5C12]
References
- Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.
- Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
- CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
- Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
- The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
- Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
- Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.
