Anti-VTN2 [1A5]
Invented at A*STAR Accelerate Technologies Pte Ltd
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![Western blotting was performed on 10 or 100ng of purified proteins; VTN2 protein (lanes 1 and 2), VTN3 protein (lanes 3 and 4) and VTN4 protein (lanes 5 and 6). Anti-VTN2 [1A5] detects the VTN4 protein, most likely due to the degree of sequence similarity.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/8b6c26ea-e14f-4653-949c-7a33541841a3.png)
![Immunofluorescence on whole-mount zebrafish embryos from two developmental stages performed using Anti-VTN2 [1A5] at a dilution of 1:1000.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/eccbccfc-9194-413a-a4f8-f3ab191d75f8.png)
![Immunofluorescence was performed on 293T cells. Left panel: pre-immune serum; Middle panel: Anti-VTN2 [1A5] at 1:500 dilution; Right panel: commercially sourced monoclonal.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f7319176-ae5f-4e36-83cb-3560f80421c0.png)
![Western blotting was performed on 10 or 100ng of purified proteins; VTN2 protein (lanes 1 and 2), VTN3 protein (lanes 3 and 4) and VTN4 protein (lanes 5 and 6). Anti-VTN2 [1A5] detects the VTN4 protein, most likely due to the degree of sequence similarity.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/8b6c26ea-e14f-4653-949c-7a33541841a3.png)
![Immunofluorescence on whole-mount zebrafish embryos from two developmental stages performed using Anti-VTN2 [1A5] at a dilution of 1:1000.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/eccbccfc-9194-413a-a4f8-f3ab191d75f8.png)
![Immunofluorescence was performed on 293T cells. Left panel: pre-immune serum; Middle panel: Anti-VTN2 [1A5] at 1:500 dilution; Right panel: commercially sourced monoclonal.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f7319176-ae5f-4e36-83cb-3560f80421c0.png)
![Image thumbnail for Anti-VTN2 [1A5]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/d29e1878-ab86-4568-86c5-358ebcfead61.png)
![Image thumbnail for Anti-VTN2 [1A5]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/8b6c26ea-e14f-4653-949c-7a33541841a3.png)
![Image thumbnail for Anti-VTN2 [1A5]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/eccbccfc-9194-413a-a4f8-f3ab191d75f8.png)
![Image thumbnail for Anti-VTN2 [1A5]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f7319176-ae5f-4e36-83cb-3560f80421c0.png)
| Catalogue Number | 152667 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | Splice variant of Zebrafish IKKg protein (154 amino acids) |
| Reactivity | Human and Zebrafish |
| Relevance | NF-κB (nuclear factor kappaB) is a transcription factor that activates numerous target genes important in mediating innate and adaptive immunity in mammals. Activation by external stimuli such as TNF leads to the activation of kinases (IKK1 and IKK2) that phosphorylate inhibitory proteins I-κB and their subsequent degradation. IKK1 and IKK2 form the catalytic subunits of a complex which includes IKKγ, the regulatory component (also known as NEMO). Mutations in NEMO are the cause of the human disorders incontinentia pigmenti and ectodermal dysplasia (ED-AID). |
| Host | Mouse |
| Immunogen | GST/His-tagged splice variant of Zebrafish IKKγ protein of 154 amino acids fusion protein |
| Positive Control | Zebrafish embryos, HEK293 cells |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Aquaculture, Epigenetics & Nuclear Signalling, Protein Degradation |
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