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Anti-ORF7a [3C9]

Info

Catalogue Number 152647
Applications IF WB
Antigen/Gene or Protein Targets SARS CoV ORF7a (also known as U122)
Reactivity Virus
Relevance Monoclonal antibody used to study the role of ORF7a within SARS coronavirus.

Background and Research Application
A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals.

ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1.
Host Mouse
Immunogen GST-7a (16-111 amino acids)
Immunogen UniProt ID J9T5H6
Subclass IgG1
Molecular Weight (kDa) 14
Myeloma Used Sp2/0-Ag14
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Overexpression of Bcl-XL blocks the induction of apoptosis by ORF7a, indicating that ORF7a may be acting at the level of or upstream from the Bcl-2 family.

One study showed that the over-expression of ORF7a inhibited cellular protein synthesis and induced the phosphorylation and activation of p38 mitogen activated protein kinase (MAPK).

Concentration
1mg/ml as standard
Research Area Cell Signaling & Signal Transduction, Neurobiology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.

Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.

Europe PMC ID: 17428862


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References: 2 entries

Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.

Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.


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