Anti-ORF7a [3C9]
Invented at A*STAR Accelerate Technologies Pte Ltd
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- References (2)
- Inventor Info
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![Western blotting on lysates prepared from SARS-CoV-infected Vero E6 cells harvested at different time points postinfection. Western blot analysis was performed to determine the expression level of SARS-CoV proteins, using anti-ORF7a [3C9], anti-3a mouse polyclonal antibody, anti-M rabbit polyclonal antibody,and anti-N mouse polyclonal antibody. An anti-actin monoclonal antibody was used to verify equal protein loading](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/8f0a4067-2418-4af7-a3c0-49705b734818.png)
![Immunofluorescence on Vero E6 cells expressing 7a using anti-ORF7a [3C9] and a FITC-conjugated anti-mouse secondary antibody or Mito-Tracker red dye which labels the mitochondria of the cell. In the merged image, the fraction of 7a protein localising to the mitochondria is indicated by the yellow signal.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9eaa314a-f3b1-428f-b514-a23d4828c21f.png)
![Western blotting on lysates prepared from SARS-CoV-infected Vero E6 cells harvested at different time points postinfection. Western blot analysis was performed to determine the expression level of SARS-CoV proteins, using anti-ORF7a [3C9], anti-3a mouse polyclonal antibody, anti-M rabbit polyclonal antibody,and anti-N mouse polyclonal antibody. An anti-actin monoclonal antibody was used to verify equal protein loading](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/8f0a4067-2418-4af7-a3c0-49705b734818.png)
![Immunofluorescence on Vero E6 cells expressing 7a using anti-ORF7a [3C9] and a FITC-conjugated anti-mouse secondary antibody or Mito-Tracker red dye which labels the mitochondria of the cell. In the merged image, the fraction of 7a protein localising to the mitochondria is indicated by the yellow signal.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9eaa314a-f3b1-428f-b514-a23d4828c21f.png)
![Image thumbnail for Anti-ORF7a [3C9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/8f0a4067-2418-4af7-a3c0-49705b734818.png)
![Image thumbnail for Anti-ORF7a [3C9]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9eaa314a-f3b1-428f-b514-a23d4828c21f.png)
| Catalogue Number | 152647 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | SARS CoV ORF7a (also known as U122) |
| Reactivity | Virus |
| Relevance |
Monoclonal antibody used to study the role of ORF7a within SARS coronavirus. Background and Research Application A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals. ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1. |
| Host | Mouse |
| Immunogen | GST-7a (16-111 amino acids) |
| Immunogen UniProt ID | J9T5H6 |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 14 |
| Myeloma Used | Sp2/0-Ag14 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Overexpression of Bcl-XL blocks the induction of apoptosis by ORF7a, indicating that ORF7a may be acting at the level of or upstream from the Bcl-2 family. One study showed that the over-expression of ORF7a inhibited cellular protein synthesis and induced the phosphorylation and activation of p38 mitogen activated protein kinase (MAPK). Concentration 1mg/ml as standard |
| Research Area | Cell Signaling & Signal Transduction, Neurobiology |
References: 2 entries
Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.
Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.
Europe PMC ID: 17428862
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References: 2 entries
Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.
Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.
Add a reference
