#152647

Anti-ORF7a [3C9]

Cat. #152647

Anti-ORF7a [3C9]

Cat. #: 152647

Unit size: 100 ug

Availability: 10-12 weeks

Target: SARS CoV ORF7a (also known as U122)

Class: Monoclonal

Application: IF ; WB

Reactivity: Virus

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: A*STAR Accelerate Technologies Pte Ltd

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-ORF7a [3C9]
  • Clone: 3C9
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 14 kDa
  • Reactivity: Virus
  • Host: Mouse
  • Application: IF ; WB
  • Description: Monoclonal antibody used to study the role of ORF7a within SARS coronavirus. Background and Research Application A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals. ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1.
  • Immunogen: GST-7a (16-111 amino acids)
  • Immunogen uniprot id: J9T5H6
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: SARS CoV ORF7a (also known as U122)
  • Molecular weight: 14 kDa
  • Target background: Monoclonal antibody used to study the role of ORF7a within SARS coronavirus. Background and Research Application A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals. ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1.

Applications

  • Application: IF ; WB

Handling

  • Format: Liquid
  • Unit size: 100 ug
  • Shipping conditions: Shipping at 4° C

References

  • Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.
  • Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.