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Anti-EBV Latent Membrane Protein 1 [LMPO24]

Invented by Prof Martin Rowe at University Of Birmingham

Info

Catalogue Number 152632
Applications FACS IHC
Antigen/Gene or Protein Targets Epstein-Barr Virus, Latent Membrane Protein 1 (EBV-LMP1)
Reactivity Virus
Relevance Monoclonal antibody which can be used to investigate EBV latency and EBV-associated malignant cells.

Background and Research Application
EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases. These include infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas.

This antibody is specific for latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV). LMP1 is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. This protein can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
Host Mouse
Immunogen Purified plasma membranes from LMP1 expressing insect cells infected with a recombinant LMP1 baculovirus
Immunogen UniProt ID P03230
Positive Control EBV transformed lymphoblastoid cell lines
Subclass IgG1 kappa
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-LMP1 has a strong and specific reactivity in immunohistochemical staining of paraffin-embedded tissues.
LMP024 helped show many LMP1-positive large lymphoid blasts.

Niedobitek et al., 1995: "The LMPO24 clone demonstrates strong and specific reactivity in immunohistochemical staining of paraffin-embedded tissues".

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cell Signaling & Signal Transduction, Immunology, Virology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Niedobitek et al. 1995. Blood. 86(2):659-65. PMID: 7605996.

IHC

Heterogeneous expression of Epstein-Barr virus latent proteins in endemic Burkitt's lymphoma.

Europe PMC ID: 7605996


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References: 2 entries

Niedobitek et al. 1995. Blood. 86(2):659-65. PMID: 7605996.

IHC

Heterogeneous expression of Epstein-Barr virus latent proteins in endemic Burkitt's lymphoma.


Add a reference