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SVCT-MI2 Cell Line

Invented by Prof Joyce Taylor-Papadimitriou at King's College London, Dr Sidney Chang at Retired Consultant

Info

Catalogue Number 152596
Antigen/Gene or Protein Targets Human oncogene EJ Harvey-ras
Host Human
Tissue Breast
Disease Keywords Breast Cancer
Relevance SVCT-MI2 was established in 1987 from epithelial cells in breast milk secretions. The cells have been transfected by microinjection using SV40 DNA and a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. After selection of G418-resistant colonies, the clone SVCT-MI2 was shown to be tumorigenic in nude mice. They also have the ability to grow anchorage-independent if cultured in soft agar.
Conditional No
Research Area Cancer, Cell Cycle, Drug Discovery & Development, Epigenetics & Nuclear Signalling
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions RPMI 1640 or DMEM + 2mM Glutamine + 10µg / ml insulin + 5µg / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Note: cells may pile up when confluent.
Cellosaurus ID CVCL_2719

References: 1 entry

Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.


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References: 1 entry

Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.


Add a reference

References: 1 entry

Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.


Add a reference


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