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Anti-CD3, Recombinant [UCH-T1®]
RECENTLY UPDATED

Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields

Info

Catalogue Number 152589
Applications FACS WB
Antigen/Gene or Protein Targets CD3
Reactivity Human
Relevance Recombinant version of CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia’s.

Background and Research Application
The CD3 complex, composed of four distinct CD3 polypeptide chains ( CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction.

This is a recombinant version of anti-CD3 UCH-T1 . UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemia’s and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events.

This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.
Host Mouse
Immunogen Human infant thymocytes and Sezary cells.
Subclass IgG1
Formulation PBS only
Concentration 1mg/ml
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology
Immunogen UniProt ID P04234
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
UCHT1 binds to an acidic region of CD3-epsilon, occluding this region form direct interaction with the T cell receptor. UCHT1 readily detects T-cell populations, in peripheral blood and lymph nodes, as CD3 is present on around 68-82% of healthy peripheral blood lymphocytes.

Recommended for the enrichment of T cell populations by cell sorting or panning.

Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

Concentration
1mg/ml as standard

References

There are 2 reference entries for this reagent.

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References: 2 entries

Original hybridoma first published in: Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.

Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.

Europe PMC ID: 6788570


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References: 2 entries

Original hybridoma first published in: Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.

Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.


Add a reference