Anti-CD3, Recombinant [UCH-T1®]
Invented by Prof Peter Beverley from Imperial College
- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||CD3|
Recombinant version of CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia’s.
Background and Research Application
The CD3 complex, composed of four distinct CD3 polypeptide chains ( CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction.
This is a recombinant version of anti-CD3 UCH-T1 . UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemia’s and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events.
This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.
|Immunogen||Human infant thymocytes and Sezary cells.|
|Research Area||Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology|
|Immunogen UniProt ID||P04234|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
UCHT1 binds to an acidic region of CD3-epsilon, occluding this region form direct interaction with the T cell receptor. UCHT1 readily detects T-cell populations, in peripheral blood and lymph nodes, as CD3 is present on around 68-82% of healthy peripheral blood lymphocytes.
Recommended for the enrichment of T cell populations by cell sorting or panning.
Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.
1mg/ml as standard
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References: 2 entries
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