Cancer Research Technology
Log in Register
Menu

Anti-CD3 [UCH-T1®]

Invented by Prof Peter Beverley at Imperial College

Info

Catalogue Number 151175
Applications FACS IHC IF IP Fn RIA WB
Antigen/Gene or Protein Targets CD3
Synonyms T3 complex
Reactivity Human
Relevance The CD3 complex, composed of five CD3 polypeptide chains, associates with the T cell antigen receptor (TCR) and is found on all mature human T lymphocytes. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events.
Host Mouse
Immunogen Human infant thymocytes and Sezary cells.
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 5% FCS.
Strain Balb/c
Notes Recommended for the enrichment of T cell populations by cell sorting or panning.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References: 18 entries

Érsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.

CD3ζ-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.

Europe PMC ID: 22798681

Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.

Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.

Europe PMC ID: 19494291

Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.

Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.

Europe PMC ID: 18796645

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Europe PMC ID: 11694532

Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.

Fn

Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.

Europe PMC ID: 10464312

Salmerón et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.

IP

A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.

Europe PMC ID: 1717585

Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.

Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.

Europe PMC ID: 6227478

Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.

Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.

Europe PMC ID: 6178042

Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.

Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.

Europe PMC ID: 6788570


Add a reference

References: 18 entries

Érsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.

CD3ζ-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.

Europe PMC ID: 22798681

Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.

Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.

Europe PMC ID: 19494291

Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.

Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.

Europe PMC ID: 18796645

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Europe PMC ID: 11694532

Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.

Fn

Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.

Europe PMC ID: 10464312

Salmerón et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.

IP

A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.

Europe PMC ID: 1717585

Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.

Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.

Europe PMC ID: 6227478

Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.

Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.

Europe PMC ID: 6178042

Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.

Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.

Europe PMC ID: 6788570


Add a reference

References: 18 entries

Érsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.

CD3ζ-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.

Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.

Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.

Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.

Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.

Fn

Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.

Salmerón et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.

IP

A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.

Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.

Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.

Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.

Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.

Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.

Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.


Add a reference


This reagent does not have any reviews at the moment.