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Anti-Involucrin, Recombinant [SY5]

Info

Catalogue Number 152587
Applications IHC IF IP WB
Antigen/Gene or Protein Targets Involucrin
Reactivity Dog, Human, Pig and Primate
Relevance Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Host Mouse
Immunogen Purified involucrin from human keratinocytes.
Subclass IgG1
Formulation PBS only
Concentration 1mg/ml
Research Area Cancer, Cell Signaling & Signal Transduction
Notes There is weak reactivity with Canine

Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

References: 2 entries

Original hybridoma first published in Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.

Europe PMC ID: 1500072


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References: 2 entries

Original hybridoma first published in Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.

Europe PMC ID: 1500072


Add a reference

References: 2 entries

Original hybridoma first published in Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.


Add a reference


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