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Anti-Involucrin [SY5]
RECENTLY UPDATED

Invented by Prof Fiona Watt at King's College London

Info

Catalogue Number 151163
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Involucrin
Reactivity Dog, Human, Pig and Primate
Relevance Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Host Mouse
Immunogen Purified involucrin from human keratinocytes.
Subclass IgG1 kappa
Positive Control MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Molecular Weight (kDa) 66-170
Myeloma Used P3X63Ag8
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes There is weak reactivity with Canine, but it does not react with Mouse.
Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
Research Area
RECENTLY UPDATED
Cancer, Cell Signaling & Signal Transduction

References: 18 entries
RECENTLY UPDATED

Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.

IF

Germline NLRP1 Mutations Cause Skin Inflammatory and Cancer Susceptibility Syndromes via Inflammasome Activation.

Europe PMC ID: 27662089

Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.

Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439.

Europe PMC ID: 27448499

A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin.

Europe PMC ID: 27079667

Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.

Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.

IHC

Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries.

Europe PMC ID: 26303436

Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.

IHC

Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.

Europe PMC ID: 24700702

Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.

MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells.

Europe PMC ID: 22331466

White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.

Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.

Europe PMC ID: 20861360

Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.

Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in HaCaT cells.

Europe PMC ID: 18633436

Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.

Europe PMC ID: 1500072


Add a reference

References: 18 entries
RECENTLY UPDATED

Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.

IF

Germline NLRP1 Mutations Cause Skin Inflammatory and Cancer Susceptibility Syndromes via Inflammasome Activation.

Europe PMC ID: 27662089

Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.

Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439.

Europe PMC ID: 27448499

A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin.

Europe PMC ID: 27079667

Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.

Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.

IHC

Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries.

Europe PMC ID: 26303436

Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.

IHC

Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.

Europe PMC ID: 24700702

Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.

MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells.

Europe PMC ID: 22331466

White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.

Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.

Europe PMC ID: 20861360

Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.

Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in HaCaT cells.

Europe PMC ID: 18633436

Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.

Europe PMC ID: 1500072


Add a reference

References: 18 entries
RECENTLY UPDATED

Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.

IF

Germline NLRP1 Mutations Cause Skin Inflammatory and Cancer Susceptibility Syndromes via Inflammasome Activation.

Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.

Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439.

A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin.

Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.

Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.

IHC

Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries.

Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.

IHC

Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.

Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.

MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells.

White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.

Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.

Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.

Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in HaCaT cells.

Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

Characterisation of eight monoclonal antibodies to involucrin.


Add a reference


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