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|Applications||FACS IHC IF IP WB|
|Antigen/Gene or Protein Targets||Involucrin|
|Reactivity||Dog, Human, Pig and Primate|
|Relevance||Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.|
|Immunogen||Purified involucrin from human keratinocytes.|
|Positive Control||MCF-7 cells. Localized to upper spinous and granular layers in normal skin.|
|Molecular Weight (kDa)||66-170|
|Recommended Growing Conditions||DMEM + 5% FCS|
There is weak reactivity with Canine, but it does not react with Mouse.
Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
|Research Area||Cancer, Cell Signaling & Signal Transduction|
References: 9 entries
Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.
Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.
Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.
Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.
Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.
Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.
White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.
Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.
Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.
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