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Anti-Involucrin [SY5]


Catalogue Number 151163
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Involucrin
Reactivity Dog, Human, Pig and Primate
Relevance Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Host Mouse
Immunogen Purified involucrin from human keratinocytes.
Subclass IgG1 kappa
Positive Control MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Molecular Weight (kDa) 66-170
Myeloma Used P3X63Ag8
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes There is weak reactivity with Canine, but it does not react with Mouse.
Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
Research Area Cancer, Cell Signaling & Signal Transduction

References: 9 entries

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Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.

Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.

Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.


Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.


Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.

White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.

Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.

Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

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