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|Antigen/Gene or Protein Targets||Extracellular domains 1 and 2 of human ICAM-1|
|Disease Keywords||Rhinovirus-related diseases|
Rhinoviruses cause the common cold, acute exacerbations of asthma and other serious respiratory diseases such as chronic obstructive pulmonary disease.
The major group (MG) of rhinovirus serotypes use the human receptor ICAM-1 for cell attachment and entry and do not bind in murine ICAM-1, thus making such disease models unsuitable for the study of rhinovirus-related diseases.
This BALB/c strain transgenic disease model carries the rhinovirus-binding extracellular domains 1 and 2 of human ICAM-1. When infected with MG-rhinovirus it develops many of the disease-related outcomes seen in human rhinovirus infections
|Production Details||An expression construct containing the CMV promoter controlled-human-mouse ICAM-1chimera was excised from pHu/MuICAM-1 and electroporated into HM-1 embryonic stem(ES) cells to create stable recombinant G418-resistant ES cell lines. Genomic DNA from EScell lines was screened for pHu/MuICAM-1-specific sequence by PCR using the primers NS25 (GGG CAG TCA CAG CTA AAA CCT) and NS 26 (TCC AGG GAG CAA AAC AACTTC T). Chimeric mice were generated by C57 BL/6 J blastocyst microinjection ofpHu/MuICAM-1 ES cell clones. Transmission of the Hu/MuICAM-1 transgene wasconfirmed by PCR analysis as described above. Positive offspring were bred onto the BALB/cbackground|
|Mouse Genetic Background/Cross History||BALB/c; C57 BL/6 bred onto the BALB/cbackground by 10 successive backcross matings of male transgenic mice to wild type BALB/cfemales|
|Research Area||Cell Signaling & Signal Transduction, Genetic Studies Tools, Immunology|
MG rhinovirus-infected transgenic BALB/c develop many of the disease-related outcomes seen in human rhinovirus infections including:
airway neutrophilic and lymphocytic inflammation
induction of various chemokines and proinflammatory cytokines
production of virus-specific antibodies
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