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HCA-7 Colony29 Cell Line

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Parental Line HCA-7
Host Human
Tissue Colon
Disease Keywords Human Colon Carcinoma
Relevance The human colon cancer cell line, HCA-7 Colony 29 is a subpopulations isolated from the HCA-7 cell line. The HCA-7 cell line is an epithelial human colon adenocarcinoma cell line established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes' stage B.Colony 29 expresses Cox-2. Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid to prostaglandins and other eicosanoids. It has been found that colonic polyps and cancers overexpress Cox-2 and that the inhibition of this enzyme by nonsteroidal anti-inflammatory drugs decreases the risk of colonic neoplasia. This cell line is useful for studying the role of Cox-2 in cancer cell biology and the investigation of colorectal epithelial cell polarity.
An interesting feature of this cell line is that it forms polarised epithelial sheets which allows examination of the trafficking and release of biomolecules in vitro.
Production Details Subpopulations isolated from the HCA-7 cell line following butyrate treatment.
Research Area Cancer, Cell Structure and Motility, Drug Discovery & Development
Growth/Phenotype Keywords Morphology: Epithelial Growth Mode: Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 10% FBS + 2mM L-Glutamine + 110mg/L Sodium Pyruvate (NaP).
Notes DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 8,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
Karyotype: Near Diploid
Products: Expresses Cox-2
Receptors: EGF. Parent HCA-7 cell line: wildtype APC.
Known mutations:Parent HCA-7 cell line: RER phenotype, p53
Cellosaurus ID CVCL_2474

References: 4 entries

Coffey et al. 1997. Proc Natl Acad Sci U S A. 94(2):657-62. PMID: 9012840.

Kirkland et al. 1985. Cancer Res. 45(8):3790-5. PMID: 4016751.

Mann et al. 2001. Gastroenterology. 120(7):1713-9. PMID: 11375952.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


Add a reference

References: 4 entries

Coffey et al. 1997. Proc Natl Acad Sci U S A. 94(2):657-62. PMID: 9012840.

Kirkland et al. 1985. Cancer Res. 45(8):3790-5. PMID: 4016751.

Mann et al. 2001. Gastroenterology. 120(7):1713-9. PMID: 11375952.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


Add a reference

References: 4 entries

Coffey et al. 1997. Proc Natl Acad Sci U S A. 94(2):657-62. PMID: 9012840.

Kirkland et al. 1985. Cancer Res. 45(8):3790-5. PMID: 4016751.

Mann et al. 2001. Gastroenterology. 120(7):1713-9. PMID: 11375952.

Marsh et al. 1993. J Pathol. 170(4):441-50. PMID: 8410493.


Add a reference


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