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|Antigen/Gene or Protein Targets||N6-methyladenosine-5'-mono-phosphate|
|Reactivity||Human, Mouse and Saccharomyces cerevisiae|
|Relevance||N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.|
|Immunogen||Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine|
|Recommended Growing Conditions||DMEM with fetal calf serum (15%), L-Glutamine (2 x 10-3 M), stable Glutamine (2 x 10-3 M), non-essential amino acid solution MEM, 2-mercaptoethanol (5 x 10-5 M)|
This antibody recognises N6-methyladenosine in both modified RNA AND DNA (see associated data).
Application Note: For immunoprecipitation the best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.
IP protocol was derived from Dominissini et al., 2013 Nat Protoc. 8(1):176-89.
|Research Area||Epigenetics & Nuclear Signalling, Metabolism|
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