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Anti-N6-methyladenosine (m6A) [17-3-4-1]

Invented by Dr Rupert Fray at University of Nottingham

Info

Catalogue Number 152130
Applications IP DB
Antigen/Gene or Protein Targets N6-methyladenosine-5'-mono-phosphate
Reactivity Human, Mouse and Saccharomyces cerevisiae
Relevance N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
Host Mouse
Immunogen Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM with fetal calf serum (15%), L-Glutamine (2 x 10-3 M), stable Glutamine (2 x 10-3 M), non-essential amino acid solution MEM, 2-mercaptoethanol (5 x 10-5 M)
Strain Balb/c
Notes This antibody recognises N6-methyladenosine in both modified RNA AND DNA (see associated data).

Application Note: For immunoprecipitation the best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.

IP protocol was derived from Dominissini et al., 2013 Nat Protoc. 8(1):176-89.
Research Area Epigenetics & Nuclear Signalling, Metabolism

References: 1 entry

Bodi et al. 2010. Nucleic Acids Res. 38(16):5327-35. PMID: 20421205.


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References: 1 entry

Bodi et al. 2010. Nucleic Acids Res. 38(16):5327-35. PMID: 20421205.


Add a reference

References: 1 entry

Bodi et al. 2010. Nucleic Acids Res. 38(16):5327-35. PMID: 20421205.


Add a reference


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