Anti-N6-methyladenosine (m6A) [17-3-4-1] mAb

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-N6-methyladenosine (m6A) [17-3-4-1] mAb
• Clone: 17-3-4-1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human ; Mouse ; Saccharomyces cerevisiae
• Host: Mouse
• Application: IP ; DB
• Description: Monoclonal antibody which binds to m6A modification found in RNA and allows for analysis of methylated transcriptomes. This can be used to investigate how m6A modifications regulates gene expression. Background and Research Application N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalysed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. This antibody was created to identify m6A containing messages within RNA and helped identify the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself as being methylated in RNA transcripts. Anti-N6-methyladenosine helped narrow down the position of m6A in IME2 as towards the 3’ end. Anti-N6-methyladenosine will allow for analysis of methylated transcriptomes, and could aid understanding in how the m6A base modification regulates gene expression.
• Immunogen: Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
• Isotype: IgG1 lambda
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: N6-methyladenosine-5'-mono-phosphate
• Target background: Monoclonal antibody which binds to m6A modification found in RNA and allows for analysis of methylated transcriptomes. This can be used to investigate how m6A modifications regulates gene expression. Background and Research Application N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalysed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. This antibody was created to identify m6A containing messages within RNA and helped identify the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself as being methylated in RNA transcripts. Anti-N6-methyladenosine helped narrow down the position of m6A in IME2 as towards the 3' end. Anti-N6-methyladenosine will allow for analysis of methylated transcriptomes, and could aid understanding in how the m6A base modification regulates gene expression.

Applications

• Application: IP ; DB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Luo et al. 2020. Plant Physiol. 182(1):332-344. PMID: 31591151.
• Lan et al. 2019. Mol Cancer. 18(1):186. PMID: 31856849.
• Barbieri et al. 2017. Nature. 552(7683):126-131. PMID: 29186125.
• Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control.
• Lannes et al. 2016. Sci Rep. 6:31563. PMID: 27539363.
• N6-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO. Jia et al. 2011. Nat Chem Biol. 7(12):885-7. PMID: 22002720.
• Bodi et al. 2010. Nucleic Acids Res. 38(16):5327-35. PMID: 20421205.
• Yeast targets for mRNA methylation.