Anti-Alkaline phosphatase [AP1B9]
Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (5)
- Inventor Info
Info
| Catalogue Number | 151885 |
| Applications | IHC |
| Antigen/Gene or Protein Targets | Calf alkaline phosphatase |
| Reactivity | Bovine and Human |
| Relevance |
Monoclonal antibody which can aid detection of ovarian and testicular cancers. Background and Research Application Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. |
| Host | Mouse |
| Immunogen | Calf Alkaline phosphatase |
| Immunogen UniProt ID | P09923 |
| Positive Control | Human tonsil |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI plus FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody can be used as a second layer APAAP reagent for immunohistology. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Cycle, Cell Type or Organelle Marker |
References: 5 entries
Hoene et al. 2020. J Biomed Mater Res A. 108(4):871-881. PMID: 31846170.
Lucke et al. 2018. J Biomed Mater Res A. 106(10):2726-2734. PMID: 30051967.
Lucke et al. 2015. Biomed Res Int. 2015:938059. PMID: 25648958.
Erlach et al. 2002. J Virol. 76(6):2857-70. PMID: 11861853.
Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus.
Europe PMC ID: 11861853
Add a reference
References: 5 entries
Hoene et al. 2020. J Biomed Mater Res A. 108(4):871-881. PMID: 31846170.
Lucke et al. 2018. J Biomed Mater Res A. 106(10):2726-2734. PMID: 30051967.
Lucke et al. 2015. Biomed Res Int. 2015:938059. PMID: 25648958.
Erlach et al. 2002. J Virol. 76(6):2857-70. PMID: 11861853.
Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus.
Add a reference
