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Anti-Alkaline phosphatase [AP1B9]
RECENTLY UPDATED

Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields

Info

Catalogue Number 151885
Applications IHC
Antigen/Gene or Protein Targets Calf alkaline phosphatase
Reactivity Bovine and Human
Relevance Monoclonal antibody which can aid detection of ovarian and testicular cancers.

Background and Research Application
Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
Host Mouse
Immunogen Calf Alkaline phosphatase
Immunogen UniProt ID P09923
Positive Control Human tonsil
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI plus FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody can be used as a second layer APAAP reagent for immunohistology.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker

References

There are 5 reference entries for this reagent.

View All References

References: 5 entries

Hoene et al. 2020. J Biomed Mater Res A. 108(4):871-881. PMID: 31846170.

Lucke et al. 2018. J Biomed Mater Res A. 106(10):2726-2734. PMID: 30051967.

Lucke et al. 2015. Biomed Res Int. 2015:938059. PMID: 25648958.

Erlach et al. 2002. J Virol. 76(6):2857-70. PMID: 11861853.

Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus.

Europe PMC ID: 11861853


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References: 5 entries

Hoene et al. 2020. J Biomed Mater Res A. 108(4):871-881. PMID: 31846170.

Lucke et al. 2018. J Biomed Mater Res A. 106(10):2726-2734. PMID: 30051967.

Lucke et al. 2015. Biomed Res Int. 2015:938059. PMID: 25648958.

Erlach et al. 2002. J Virol. 76(6):2857-70. PMID: 11861853.

Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus.


Add a reference