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Anti-NSE [NSEP2]
RECENTLY UPDATED

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151867
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Gamma-isozyme of human enolase (NSE)
Reactivity Human
Relevance Neuron specific enolase (NSE, or ?-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Host Mouse
Immunogen Immunogen: ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa’s 271-285 - TGDQLGALYQDFVRD
Subclass IgG1 kappa
Positive Control Formalin-fixed, paraffin-embedded nerve tissue sectionswestern blot: ?-isozyme of human enolase; 50-100 ng per lane
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain Balb/c
Notes Not reactive with -isozyme of enolase.
Research Area
RECENTLY UPDATED
Cancer, Cell Type or Organelle Marker, Developmental Biology, Metabolism, Neurobiology, Stem Cell Biology

References: 2 entries

Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.


Add a reference

References: 2 entries

Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.


Add a reference

References: 2 entries

Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.


Add a reference


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