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Monoclonal Antibodies

Anti-NSE [NSEP1]

Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Lysates were separated on SDS-PAGE and probed with Anti-NSE [NSE-P1]. Lane 1: SH-SY5Y, Lane 2: NT2/D1.
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Catalogue Number 151866
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Gamma-isozyme of human enolase (NSE)
Reactivity Human
Relevance Neuron specific enolase (NSE, or gamma-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P1: aa’s 416-433 - LGDEARFAGHNFRNPSVL
Positive Control IHC: formalin-fixed, paraffin-embedded nerve tissue sectionswestern blot: ?-isozyme of human enolase; 50-100 ng per lane
Subclass IgG1 kappa
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain Balb/c
Notes Not reactive with -isozyme of enolase
Research Area Cancer, Cell Type or Organelle Marker, Developmental Biology, Metabolism, Neurobiology, Stem Cell Biology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

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Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.

Europe PMC ID: 8254105

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Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.

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A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences.

Europe PMC ID: 1629611

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References: 4 entries

Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.

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Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.

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Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.

View  

A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences.

View  

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