Anti-RSV Nucleoprotein VP41 [4-18]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
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| Catalogue Number | 151852 |
| Applications | ELISA IF WB |
| Antigen/Gene or Protein Targets | Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41 |
| Reactivity | Virus |
| Relevance |
Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B. |
| Host | Mouse |
| Immunogen | Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins. |
| Positive Control | Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells |
| Subclass | IgG2a |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
| Strain | Balb/c |
| Research Area | Virology |
References: 2 entries
Gimenez et al. 1987. J Gen Virol. 68 ( Pt 5):1267-75. PMID: 3572364.
Immunoblot analysis of the human antibody response to respiratory syncytial virus infection.
Europe PMC ID: 3572364
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References: 2 entries
Gimenez et al. 1987. J Gen Virol. 68 ( Pt 5):1267-75. PMID: 3572364.
Immunoblot analysis of the human antibody response to respiratory syncytial virus infection.
Add a reference
