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|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41|
Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.
There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA.
Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.
|Immunogen||Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins.|
|Positive Control||Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).|
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