Anti-RSV Nucleoprotein VP41 [4-18]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-RSV Nucleoprotein VP41 [4-18]
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Virus
• Host: Mouse
• Application: ELISA ; IF ; WB
• Description: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.
• Immunogen: Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins.
• Isotype: IgG2a kappa
• Myeloma used: P3X63Ag8.653
• Recommended controls: Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells

Target Details

• Target: Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41
• Target background: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.

Applications

• Application: ELISA ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Gimenez HB et al. J Gen Virol. 1987 May
• 68 ( Pt 5):1267-75. PMID: 3572364