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Catalogue Number 151847
Antigen/Gene or Protein Targets
RECENTLY UPDATED
mST6-Gal-NAc and MUC1
Parental Line E3
Host Mouse
Tissue Breast
Disease Keywords Carcinoma
Model Knock-In
Relevance The E3 STn Cell Line is a mouse mammary carcinoma cell line E3 which expresses human MUC1 and Sialyl-Tn. Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn (STn) is expressed by 25–30% of breast carcinomas but its expression on normal tissue is highly restricted. Sialyl-Tn is an O-linked disaccharide that can be carried on various glycoproteins. One such glycoprotein MUC1 is expressed by the vast majority of breast carcinomas. Both STn and MUC1 have been considered as targets for immunotherapy of breast cancer patients.
Production Details E3 cell line stably transfected with murine ST6GalNAc I leading to expression of the MUC1 STn epitope. The E3 cell lines were developed from the 410.4 cell line by transfection of the hygromycin resistance gene with the MUC1 gene. Calcium phosphate transfection of E3 cells was performed using the pbabe-neo gene with or without the cDNA encoding murine B7.1. Stable transfection of murine ST6GalNAc I was performed to obtain the cell line E3-STn.
Research Area
RECENTLY UPDATED
Cancer, Drug Discovery & Development, Immunology
Recommended Growing Conditions Dulbecco’s E4 medium containing 600 µg/ml G418, 200 µg/ml hygromycin and 10% fetal calf serum (FCS)
Notes
RECENTLY UPDATED
E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn
Cellosaurus ID CVCL_W346

References: 1 entry

Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.


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References: 1 entry

Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.


Add a reference

References: 1 entry

Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.


Add a reference


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