Cat. #151847
E3 STn Cell Line
Cat. #: 151847
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Mouse
Tissue: Breast
Disease: Cancer
Model: Knock-In
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joyce Taylor-Papadimitriou ; Joy Burchell
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: E3 STn Cell Line
- Tool sub type: Continuous
- Parental cell: E3
- Organism: Mouse
- Tissue: Breast
- Disease: Cancer
- Model: Knock-In
- Conditional: Yes
- Description: The E3 STn Cell Line is a mouse mammary carcinoma cell line E3 which expresses human MUC1 and Sialyl-Tn. Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn (STn) is expressed by 2530% of breast carcinomas but its expression on normal tissue is highly restricted. Sialyl-Tn is an O-linked disaccharide that can be carried on various glycoproteins. One such glycoprotein MUC1 is expressed by the vast majority of br...
- Production details: E3 cell line stably transfected with murine ST6GalNAc I leading to expression of the MUC1 STn epitope. The E3 cell lines were developed from the 410.4 cell line by transfection of the hygromycin resistance gene with the MUC1 gene. Calcium phosphate transfection of E3 cells was performed using the pbabe-neo gene with or without the cDNA encoding murine B7.1. Stable transfection of murine ST6GalNAc I was performed to obtain the cell line E3-STn.
- Biosafety level: 1
- Additional notes: E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn
- Recommended controls: E3 parental line
- Cellosaurus id: CVCL_W346
Target Details
- Target: mST6-Gal-NAc and MUC1
Applications
- Application notes: E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn
Handling
- Format: Frozen
- Growth medium: Dulbecco's E4 medium containing 600 ??g/ml G418, 200 ??g/ml hygromycin and 10% fetal calf serum (FCS)
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
- Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
