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2fTGH-U2A Cell Line

Info

Catalogue Number 151807
Antigen/Gene or Protein Targets IFN signalling mutant U2A
Parental Line HT 1080
Host Human
Disease Keywords Fibrosarcoma
Model Mutant
Relevance The 2fTGH-U2A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and span multiple members of the IFMy pathway. These cell lines are be useful for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.

The following cell lines are part of the group of IFN signalling mutants: U4C, U2A, U3A, 2FTGH, U6A, U5A. Each containing a different mutation in the IFN signalling pathway.
Production Details Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.
Research Area Cell Signaling & Signal Transduction, Immunology
Recommended Growing Conditions Parental 2fTGH and mutant cell lines can be grown in DMEM with 10% FCS.
Positive Control The wild type 2FTGH human fibrosarcoma as a positive control together with the U5a and U3a IFNB resistant cell lines.
Cellosaurus ID CVCL_M019

References: 18 entries

Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.

Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.

Europe PMC ID: 18178840

Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.

Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.

Europe PMC ID: 15212709

Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.

Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection.

Europe PMC ID: 10662616

Leaman et al. 1998. Proc Natl Acad Sci U S A. 95(16):9442-7. PMID: 9689099.

A mutant cell line defective in response to double-stranded RNA and in regulating basal expression of interferon-stimulated genes.

Europe PMC ID: 9689099

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.

Europe PMC ID: 9001223

Rani et al. 1996. J Biol Chem. 271(37):22878-84. PMID: 8798467.

Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha.

Europe PMC ID: 8798467

Lutfalla et al. 1995. EMBO J. 14(20):5100-8. PMID: 7588638.

Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.

Europe PMC ID: 7588638

McKendry et al. 1991. Proc Natl Acad Sci U S A. 88(24):11455-9. PMID: 1837150.

High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.

Europe PMC ID: 1837150

Pellegrini et al. 1989. Mol Cell Biol. 9(11):4605-12. PMID: 2513475.

Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.

Europe PMC ID: 2513475


Add a reference

References: 18 entries

Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.

Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.

Europe PMC ID: 18178840

Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.

Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.

Europe PMC ID: 15212709

Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.

Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection.

Europe PMC ID: 10662616

Leaman et al. 1998. Proc Natl Acad Sci U S A. 95(16):9442-7. PMID: 9689099.

A mutant cell line defective in response to double-stranded RNA and in regulating basal expression of interferon-stimulated genes.

Europe PMC ID: 9689099

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.

Europe PMC ID: 9001223

Rani et al. 1996. J Biol Chem. 271(37):22878-84. PMID: 8798467.

Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha.

Europe PMC ID: 8798467

Lutfalla et al. 1995. EMBO J. 14(20):5100-8. PMID: 7588638.

Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.

Europe PMC ID: 7588638

McKendry et al. 1991. Proc Natl Acad Sci U S A. 88(24):11455-9. PMID: 1837150.

High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.

Europe PMC ID: 1837150

Pellegrini et al. 1989. Mol Cell Biol. 9(11):4605-12. PMID: 2513475.

Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.

Europe PMC ID: 2513475


Add a reference

References: 18 entries

Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.

Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.

Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.

Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.

Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.

Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection.

Leaman et al. 1998. Proc Natl Acad Sci U S A. 95(16):9442-7. PMID: 9689099.

A mutant cell line defective in response to double-stranded RNA and in regulating basal expression of interferon-stimulated genes.

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.

Rani et al. 1996. J Biol Chem. 271(37):22878-84. PMID: 8798467.

Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha.

Lutfalla et al. 1995. EMBO J. 14(20):5100-8. PMID: 7588638.

Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.

McKendry et al. 1991. Proc Natl Acad Sci U S A. 88(24):11455-9. PMID: 1837150.

High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.

Pellegrini et al. 1989. Mol Cell Biol. 9(11):4605-12. PMID: 2513475.

Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.


Add a reference


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