Anti-Tdp2, Polyclonal [Tdp2]
Invented at Cancer Research UK Manchester Institute
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- References (2)
- Inventor Info
Info
| Catalogue Number | 151785 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Tdp2 |
| Reactivity | Human |
| Relevance | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. |
| Host | Rabbit |
| Immunogen | Recombinant human protein with his tag |
| Molecular Weight (kDa) | 49 |
| Notes | Suggested Dilutions: 1:1000 - 1:10000 |
| Positive Control | A549 |
| Research Area | Cancer, Epigenetics & Nuclear Signalling |
References: 2 entries
Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181.
Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.
Europe PMC ID: 23416181
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References: 2 entries
Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181.
Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.
Add a reference
