Anti-Tdp2 [Tdp2]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-Tdp2 [Tdp2]
• Clone: Tdp2
• Tool sub type: Primary antibody
• Class: Polyclonal
• Conjugation: Unconjugated
• Molecular weight: 49 kDa
• Reactivity: Human
• Host: Rabbit
• Application: WB
• Description: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
• Immunogen: Recombinant human protein with his tag
• Recommended controls: A549

Target Details

• Target: Tdp2
• Molecular weight: 49 kDa
• Tissue cell line specificity: A549
• Target background: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Applications

• Application: WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181.
• Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.