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Anti-pTpY Cdk1 [CP3.2]

Invented by Julian Gannon at The Francis Crick Institute

Info

Catalogue Number 151684
Applications ELISA WB
Antigen/Gene or Protein Targets Cdk1
Synonyms Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; Cell Cycle Controller CDC2; CDKN1
Reactivity Mammalian and Xenopus laevis
Relevance Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer
Host Mouse
Immunogen Peptide - EKIGEGpTpYGVVYKGC - pT represents phosphorylated Thr14 and pY represents phosphorylated Tyr15
Subclass IgG1
Positive Control Cultured cell line
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions 10% FCS
Research Area Cancer, Cell Cycle

References: 1 entry

Trickey et al. 2013. J Biol Chem. 288(2):928-37. PMID: 23195958.


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References: 1 entry

Trickey et al. 2013. J Biol Chem. 288(2):928-37. PMID: 23195958.


Add a reference

References: 1 entry

Trickey et al. 2013. J Biol Chem. 288(2):928-37. PMID: 23195958.


Add a reference


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