Anti-pTpY Cdk1 [CP3.2]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-pTpY Cdk1 [CP3.2]
• Alternate name: Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; Cell Cycle Controller CDC2; CDKN1
• Clone: CP3.2
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Mammalian ; Xenopus laevis
• Host: Mouse
• Application: ELISA ; WB
• Description: Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer
• Immunogen: Peptide - EKIGEGpTpYGVVYKGC - pT represents phosphorylated Thr14 and pY represents phosphorylated Tyr15
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Cultured cell line

Target Details

• Target: Cdk1
• Tissue cell line specificity: Cultured cell line
• Target background: Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer

Applications

• Application: ELISA ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Trickey et al. 2013. J Biol Chem. 288(2):928-37. PMID: 23195958.
• Anaphase-promoting complex/cyclosome-mediated proteolysis of Ams2 in the G1 phase ensures the coupling of histone gene expression to DNA replication in fission yeast.