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Gamma2A Jak2 Cell Line

Info

Catalogue Number 151681
Antigen/Gene or Protein Targets Gamma2A Jak2
Parental Line HT 1080
Host Human
Disease Keywords Fibrosarcoma
Model Transgenic
Relevance The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase domains.
Production Details The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2.
Research Area Cell Signaling & Signal Transduction, Immunology
Recommended Growing Conditions DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin
Cellosaurus ID CVCL_1D51

References: 3 entries

Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.


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References: 3 entries

Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.


Add a reference

References: 3 entries

Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.


Add a reference


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